Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal
SIGMA/A7058 - clone HIS-1, purified from hybridoma cell culture
Synonym: Monoclonal Anti-polyHistidine; Monoclonal 6 His epitope tag; Monoclonal 6xHis-tag; Monoclonal HHHHHH epitope tag; Monoclonal Hexa His tag; Monoclonal His-tag; Monoclonal His6 tag; Monoclonal Histidine tagged; Monoclonal Poly-His-tag
Product Type: Chemical
Western Blotting Lysates of Escherichia coli induced to express a 6xHis tagged protein were separated on SDS-PAGE. The antibody was probed and developed with Monoclonal Anti-polyHistidine-Peroxidase antibody produced in Mouse, Clone: HIS-1 (Cat. No. A7058). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HIS-1, monoclonal |
| concentration | 5-11 mg/mL |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG2a |
| packaging | vial of 0.5 mL |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein |
| Application: | Antibody suitable for immunoblotting. Working dilution 1:20 Also suitable for dot blot assays and ELISA |
| Biochem/physiol Actions: | The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors. |
| General description: | The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors. |
| Immunogen: | recombinant polyHistidine tagged fusion protein. |
| Legal Information: | This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Preparation Note: | The antibody conjugate should be reconstituted with 0.5 ml deionized water. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |