Monoclonal Anti-Albumin antibody produced in mouse
SIGMA/A6684 - clone HSA-11, ascites fluid
Synonym: Anti-AFP
MDL Number: MFCD00162390
Product Type: Chemical
Western Blotting Human serum were separated on SDS-PAGE and probed with Monoclonal Anti-Albumin antibody produced in Mouse, Clone: HSA-11 (Cat. No. A6684). The antibody was developed using Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:5,000 antibody 2. 1:10,000 antibody 3. Negative Control
ELISA Monoclonal Anti-Albumin antibody produced in Mouse, Clone: HSA-11 (Cat. No. A6684), followed by 1:500 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Albumin by indirect ELISA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HSA-11, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | turkey, feline, hamster, pigeon, rat, marmoset, pig, bovine, canine, donkey, guinea pig, rabbit, chicken, sheep, goat |
| species reactivity | gibbon, baboon, human, rhesus monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: 1:10,000 | |
| western blot: 1:5,000 | |
| UniProt accession no. | P02768  |
| Application: | Monoclonal Anti-Albumin antibody produced in mouse has been used in: • immunofluorescence • staining • double staining • enzyme-linked immunosorbent assay (ELISA) • immunoblot • immunohistochemistry |
| Biochem/physiol Actions: | Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes . |
| Biochem/physiol Actions: | High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Oxidative damage of albumin is associated with advanced liver disease. |
| Biochem/physiol Actions: | The antibody is specific for human albumin in serum and plasma and recognizes an epitope resistant to reduction or denaturation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Albumin (mouse IgG2a isotype) is derived from the HSA-11 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Albumin, the major protein produced by the liver, represents more than half the total protein in human serum. Many other body fluids also contain albumin. |
| General description: | Serum albumin is the major protein component of blood and is produced in the liver. It regulates colloid osmotic blood pressure, transportation of a variety of substances, blood pH and provides an endogenous source of amino acids. Monoclonal anti-albumin antibody (diluted 1:100) can be used in double staining to localize hepatocytes differentiated from human umbilical mesenchymal stem cells (HUMSCs) . Offering specificity for human serum albumin, this product is ideal for identification of human blood stains or other body fluids (e.g., seminal fluid, milk, urine, saliva, vaginal secretions). Mouse monoclonal anti-albumin antibody reacts specifically with denatured and reduced human serum albumin and cross reacts with rhesus, gibbon and baboon serum albumins. The product has shown no cross reactivity with bovine, cat, chicken, dog, donkey, goat, guinea pig, hamster, marmoset, pig, pigeon, rabbit, rat, sheep, or turkey serum albumin or with chicken and turkey egg albumin. |
| Immunogen: | Human serum albumin |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |