N-Acetylneuraminic Acid Aldolase from microorganisms
SIGMA/A6680 - lyophilized powder, ≥20 units/mg protein (biuret)
Synonym: N-Acetylneuraminate Pyruvate Lyase; N-Acetylneuraminic Acid Lyase; NANA Aldolase; Sialic Aldolase
CAS Number: 9027-60-5
MDL Number: MFCD00131656
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
Figure 2. 37 °C, 5-min. reaction in 50 mM buffer solution: pH 5.0–6.0, acetate; pH 6.0–9.0, K-phosphate. The enzyme activity was assayed by the 2.4-dinitrophenyl-hydradine method.
Figure 3. Temperature activity; 5-min. reaction in 50 mM K-phosphate buffer, pH 7.5. The enzyme activity was assayed by the 2.4-dinitrophenyl-hydrazine method.
Figure 4. pH Stability; 10 °C, 25-hr treatment with 50 mM buffer solution: pH 4.0–6.0, acetate; pH 6.0–9.0, K-phosphate; pH 9.0–10.0, borate
Figure 5. Thermal stability; 30-min. treatment with 50 mM K-phosphate buffer, pH 7.5.
| form | lyophilized powder |
| mol wt | ~98 kDa |
| Quality Level | 200  |
| specific activity | ≥20 units/mg protein (biuret) |
| storage temp. | −20°C |
| Application: | This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when coupled with the related enzymes in clinical analysis. For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid. |
| Application: | Used in the Sialic Acid Quantification Kit, SIALIC-Q |
| General description: | Isoelectric point: 4.6 ± 0.1 Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid) Structure: 3 subunits (approx. 35,000) per mol of enzyme Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+ Optimum pH: 7.5– 8.0 Optimum temp: 70°C pH Stability: pH 6.0–9.0 (10°C, 25hr) Thermal stability: Below 65°C (pH 7.5, 30 min) |
| Other Notes: | One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C. |
| Packaging: | 25 units in glass bottle |
| Physical form: | Lyophilized powder containing mannitol and EDTA |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20 units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.1.3.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |