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Adenosine 5′-triphosphate disodium salt solution

SIGMA/A6559 - Crystalline ATP, HPLC purified, aqueous solution for RNA transcription

Synonym: ATP

CAS Number: 987-65-5
MDL Number: MFCD00080339
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-A6559-25UMO 25 µmol
$131.00
1/EA
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assay ≥95%
biological source yeast
concentration 100 mM in H2O (adjusted to pH 7 with Trizma®
base)
foreign activity DNase, RNase, none detected
form liquid
InChI 1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1
InChI key TTWYZDPBDWHJOR-IDIVVRGQSA-L
Quality Level 300 
shipped in dry ice
SMILES string [Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O
storage temp. −20°C
Application: Adenosine 5′-triphosphate disodium salt solution has been used:
• as a standard to evaluate the extracellular ATP release from marginal cells of stria vascular
• as a component of the internal pipette solution to establish a recording electrode to study neurite stretching
• in in vitro VPS34 (catalytic subunit of the phosphatidylinositol 3-kinase (PtdIns3K) complex) activity assay
• as a component of lysis and resealing buffers for ATP studies
• in spectrophotometric measurement of H+ transport in fluorescein isothiocyanate (FITC)-dextran loaded A549 (adenocarcinomic human alveolar basal epithelial cells) and HeLa cells
• to induce pyroptosis in lipopolysaccharide treated fibroblast-like synoviocytes (FLSs)
Biochem/physiol Actions: Adenosine 5′-triphosphate (ATP) is an important energy currency in all living organisms. It possesses high phosphate transfer potential. ATP is involved in several biological processes such as membrane transport, muscle contraction and synthesis, and degradation of biological molecules. It plays a role as an intracellular and extracellular signaling molecule in certain cellular processes such as cell motility, organ development, neurotransmission, and insulin secretion.
General description: Adenosine 5′-triphosphate disodium salt solution is suitable for use in molecular biology applications such as kinase reactions or DNA-dependent RNA polymerase transcription.
Legal Information: Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95%
Storage Temp. −20°C
UNSPSC 41106305

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