Aprotinin from bovine lung
SIGMA/A6106 - BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)
Synonym: BPTI; Bovine pancreatic trypsin inhibitor; Trasylol; Trypsin inhibitor (basic)
CAS Number: 9087-70-1
Empirical Formula (Hill Notation): C284H432N84O79S7
Molecular Weight: 6511.44
EC Number: 232-994-9
MDL Number: MFCD00130541
Linear Formula: C284H432N84O79S7
Product Type: Chemical
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung. Aprotinin is a single peptide chain with three disulfide bonds. Molecular Weight: ~6511
SDS-PAGE (4-20%) of aprotinin (Cat. No. A6106) under reducing conditions and stained with Coomassie blue.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A6106-100MG
| assay | ≥98% (SDS-PAGE) |
| biological source | bovine lung |
| form | powder |
| InChI | 1S/C284H432N84O79S7/c1-21 |
| InChI key | ZPNFWUPYTFPOJU-UHFFFAOYSA |
| mol wt | ~6,500 |
| product line | BioUltra |
| Quality Level | 300  |
| SMILES string | S1SCC2NC(=O)CNC(=O)CNC(=O |
| solubility | H2O: 5 mg/mL, clear to very slightly hazy |
| specific activity | 3-8 TIU/mg solid |
| storage temp. | 2-8°C |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | P00974  |
| Application: | Aprotinin is largely used as an inhibitor of trypsin. |
| Biochem/physiol Actions: | Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges. |
| Other Notes: | One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl- |
| Other Notes: | One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-argini |
| Packaging: | 1 mg in glass bottle |
| Packaging: | 25, 100 mg in poly bottle |
| Preparation Note: | A further purification of A1153 to yield a product with slightly higher activity and purity |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| activity | specific activity: 3-8 TIU/mg solid |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |