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Ala-Ala-Phe-7-amido-4-methylcoumarin

SIGMA/A3401 - protease substrate

Synonym: H-Ala-Ala-Phe-AMC

CAS Number: 62037-41-6
Empirical Formula (Hill Notation): C25H28N4O5
Molecular Weight: 464.51
MDL Number: MFCD00070149
Linear Formula: C25H28N4O5
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-A3401-25MG 25 mg
 $174.00
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assay ≥98% (TLC)
form powder
InChI 1S/C25H28N4O5/c1-14-11-22(30)34-21-13-18(9-10-19(14)21)28-25(33)20(12-17-7-5-4-6-8-17)29-24(32)16(3)27-23(31)15(2)26/h4-11,13,15-16,20H,12,26H2,1-3H3,(H,27,31)(H,28,33)(H,29,32)
InChI key FVRLYIFIDKXFHU-UHFFFAOYSA-N
Quality Level 200 
SMILES string CC(N)C(=O)NC(C)C(=O)NC(Cc1ccccc1)C(=O)Nc2ccc3C(C)=CC(=O)Oc3c2
solubility ethanol: 20 mg/mL, clear, colorless to light yellow
storage temp. 2-8°C
Application: Ala-Ala-Phe-7-amido-4-methylcoumarin has been used:
• in the preparation of the reaction mixture for enzymatic assays
• to initiate the enzyme reaction in tripeptidyl peptidase-1 (TPP1) enzyme activity assay
• to initiate the assay reaction in lysosomal hydrolases activity assay
General description: Ala-Ala-Phe-7-amido-4-methylcoumarin is a fluorogenic substrate for the proteolytic activity. It is a positively charged substrate molecule.
General description: Substrate for chymotrypsin and tripeptidyl peptidaseII.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98% (TLC)
Storage Temp. 2-8°C
UNSPSC 12352204

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