DEAE–Sephadex® A-25 chloride form
SIGMA/A25120
Synonym: DEAE Sephadex®; Sephadex A 25,2-
CAS Number: 12609-80-2
MDL Number: MFCD00132778
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A25120-50G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A25120-10G
| bead size | 40-125 μm (dry) |
| capacity | 3-4 meq/g ion exchange capacity |
| compatibility | mode of use mixed weak and strong anion exchange chromatography |
| form | powder |
| matrix | cross-linked dextran |
| matrix active group | functional group |
| operating pH | 2-9 |
| pore size | ~30,000 Da exclusion limit |
| Quality Level | 200  |
| technique(s) | activity assay: suitable |
| Application: | DEAE-Sephadex® A-25 has been used: • to form DEAE-Sephadex A-25 (formate form) for glucosinolates (GLS) extraction using horseradish plants • in the estimation of GLS content in Chinese kale sprouts • production and purification of endozepine from astrocytes • to identify and label an insulin-activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients |
| Features and Benefits: | • Weak anion exchanger • Suitable for batch techniques • High binding capacities • Non-reactive to commonly used aqueous buffers additives such as 8 M urea and 6 M guanidine hydrochloride |
| General description: | The Sephadex® ion exchangers contain a cross-linked dextran matrix with functional groups. These groups are linked by stable ether linkages to the glucose units in the matrix. DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. A25120-100G′s updated product number is GE17-0170-01 A25120-500G′s updated product number is GE17-0180-02 |
| Legal Information: | DEAE Sephadex is a registered trademark of Cytiva |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Packaging: | 10, 50, 100, 500 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 47101511 |