Triton^™ X-100 solution

SIGMA/93443 - BioUltra, Molecular Biology, ~10% in H₂O

Synonym: Triton^™ X-100; 4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution; t-Octylphenoxypolyethoxyethanol; Polyethylene glycol tert-octylphenyl ether

CAS Number: 9036-19-5
Empirical Formula (Hill Notation): (C₂H₄O)nC₁₄H₂₂O
MDL Number: MFCD00128254
Linear Formula: t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-93443-100ML	100 mL	$148.00 1/EA	Add To Favorites
45-93443-500ML	500 mL	$517.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

absorption	cut-off at 310 nm
aggregation number	100-155
cation traces	Al: ≤1 mg/kg
	Ba: ≤1 mg/kg
	Bi: ≤1 mg/kg
	Ca: ≤5 mg/kg
	Cd: ≤1 mg/kg
	Co: ≤1 mg/kg
	Cr: ≤1 mg/kg
	Cu: ≤1 mg/kg
	Fe: ≤1 mg/kg
	K: ≤100 mg/kg
	Li: ≤1 mg/kg
	Mg: ≤1 mg/kg
	Mn: ≤1 mg/kg
	Mo: ≤1 mg/kg
	Na: ≤100 mg/kg
	Ni: ≤1 mg/kg
	Pb: ≤1 mg/kg
	Sr: ≤1 mg/kg
	Zn: ≤1 mg/kg
CMC	0.2-0.9 mM (20-25°C)
	0.23 mM
concentration	~10% in H₂O
density	1.01 g/mL at 20 °C
	1.070 g/cm³
description	non-ionic
form	liquid
grade	Molecular Biology
HLB	13.5
impurities	DNases, none detected
	insoluble matter, passes filter test
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key	HNLXNOZHXNSSPN-UHFFFAOYSA-N
mol wt	average mol wt 625
	micellar avg mol wt 80,000
mp	6 °C
pH	6.5-8.5 (25 °C)
product line	BioUltra
Quality Level	200
refractive index	n20/D 1.349±0.002
SMILES string	CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
solubility	water: soluble
transition temp	cloud point 65 °C
	pour point ~7 °C

Application:	Triton^™ X-100 has been used:• for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry • As a component of lysis buffer used for homogenization of cells • As a component of blocking buffer in immunofluorescence staining • For the preparation of cells in double fluorescence staining
Biochem/physiol Actions:	Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Features and Benefits:	• Suitable for Molecular Biology and Biochemical Research • Free from DNase, RNase, NICKase and protease • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications. • High purity product for research applications • Tested for the presence of Anionic and Cationic traces
General description:	Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Legal Information:	Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Other Notes:	For additional information on our range of Biochemicals , please complete this form .

Symbol	GHS05,GHS09
Signal word	Danger
Hazard statements	H315 - H318 - H410
Precautionary statements	P273 - P280 - P302 + P352 - P305 + P351 + P338 + P310
Risk Statements	52/53
Safety Statements	60
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	483.8 °F - closed cup
Flash Point(C)	251 °C - closed cup

mp	6 °C
Density	1.01 g/mL at 20 °C; 1.070 g/cm³
Refractive Index	n20/D 1.349±0.002
UNSPSC	12161900

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