Trizma® base
SIGMA/93352 - ≥99.0% (T)
Synonym: 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| assay | ≥99.0% (T) |
| bp | 219-220 °C/10 mmHg (lit.) |
| cation traces | Ca: ≤10 mg/kg |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| description | aminopeptidase substrate |
| form | crystalline |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
| loss | ≤1% loss on drying, 110 °C |
| mp | 167-172 °C (lit.) |
| 168-172 °C | |
| pH | 10.5-12.0(4 m in water, 25 °C) |
| pKa (25 °C) | 8.1 |
| product line | BioChemika |
| Quality Level | 200  |
| SMILES string | NC(CO)(CO)CO |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| useful pH range | 7-9 |
| Application: | Trizma® base was used as buffer for the following studies: • Electrophoretic transfer for the specific identification of isozymes of starch debranching enzyme, α-amylase and 9-amylase. • Electrophoretic separation of lipoproteins in polyacrylamide gels. • Preparation of TRIS buffer having pH 8. It may be used to compose DN buffer for DNA nick-end labeling of tissue sections. |
| General description: | Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (T) |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.); 168-172 °C |
| UNSPSC | 12352104 |