Trizma® base
SIGMA/93350 - 99.7% (T), puriss. p.a.
Synonym: 2-
CAS Number: 77-86-1
Empirical Formula (Hill Notation): C4H11NO3
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH2C(CH2OH)3
Product Type: Chemical
| λ | 10 % in H2O |
| anion traces | chloride (Cl-): ≤5 mg/kg |
| sulfate (SO42-): ≤5 mg/kg | |
| assay | 99.7% (T) |
| bp | 219-220 °C/10 mmHg (lit.) |
| cation traces | Ca: ≤10 mg/kg |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| description | aminopeptidase substrate |
| form | crystalline |
| grade | puriss. p.a. |
| ign. residue | ≤0.01% (as SO4) |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7) |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA |
| mp | 167-172 °C (lit.) |
| 168-172 °C | |
| pH | 10.5-11.5 (25 °C, 10% in H2O) |
| pKa (25 °C) | 8.1 |
| Quality Level | 200  |
| SMILES string | NC(CO)(CO)CO |
| solubility | water: soluble (678 g/l at 20 °C) |
| useful pH range | 7-9 |
| UV absorption | λ: 230 nm Amax: ≤0.6 |
| λ: 260 nm Amax: ≤0.05 | |
| λ: 280 nm Amax: ≤0.04 | |
| λ: 430 nm Amax: ≤0.01 |
| Application: | Tris base has been used: • In the preparation of TAE buffer for TAE agarose gel used to run PCR products by gel electrophoresis • As a component of sample buffer for SDS-PAGE • In the preparation of IEC (ion-exchange chromatography) buffer |
| General description: | Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight. |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Easily compare specifications for Trizma products with the Trizma specification table . |
| Other Notes: | The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 99.7% (T) |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.); 168-172 °C |
| UNSPSC | 12352104 |