Trichloroacetic acid
SIGMA/91228 - BioUltra, ≥99.5% (T)
Synonym: TCA
CAS Number: 76-03-9
Empirical Formula (Hill Notation): C2HCl3O2
Molecular Weight: 163.39
EC Number: 200-927-2
MDL Number: MFCD00004177
Linear Formula: Cl3CCOOH
Product Type: Chemical
| λ | 0.5 M in H2O |
| anion traces | chloride (Cl-): ≤30 mg/kg |
| nitrate (NO3-): ≤20 mg/kg | |
| phosphate (PO43-): ≤10 mg/kg | |
| sulfate (SO42-): ≤100 mg/kg | |
| assay | ≥99.5% (T) |
| bp | 196 °C (lit.) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| density | 1.62 g/mL at 25 °C (lit.) |
| form | crystals |
| flakes | |
| grade | biotech. grade |
| ign. residue | ≤0.05% |
| impurities | insoluble matter, passes filter test |
| InChI | 1S/C2HCl3O2/c3-2(4,5)1(6) |
| InChI key | YNJBWRMUSHSURL-UHFFFAOYSA |
| mp | 54-58 °C (lit.) |
| pH | <1.0 (25 °C, 0.5 M in H2O) |
| product line | BioUltra |
| Quality Level | 200  |
| refractive index | n |
| SMILES string | OC(=O)C(Cl)(Cl)Cl |
| solubility | H2O: 0.5 M at 20 °C, clear, colorless |
| UV absorption | λ: 260 nm Amax: 1.60 |
| λ: 280 nm Amax: 0.15 | |
| vapor density | <1 (vs air) |
| vapor pressure | 1 mmHg ( 51 °C) |
| Application: | Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy. |
| Application: | Trichloroacetic acid (TCA) has been used: • to determine its effect on avian embryos during an exposure window encompassing cardiac specification • as a cell fixative • to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay • for the precipitation of acidic ribosomal proteins (RPs) |
| General description: | Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation. |
| Other Notes: | Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye. |
| Symbol |    GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H314 - H335 - H410 |
| Precautionary statements | P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | C,N |
| Risk Statements | 35-50/53 |
| Safety Statements | 26-36/37/39-45-60-61 |
| RIDADR | UN 1839 8 / PGII |
| WGK Germany | WGK 2 |
| Flash Point(F) | > 235.4 °F - closed cup |
| Flash Point(C) | > 113 °C - closed cup |
| Purity | ≥99.5% (T) |
| bp | 196 °C (lit.) |
| mp | 54-58 °C (lit.) |
| Density | 1.62 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12352101 |