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MTT Formazan

SIGMA/88417 - MTT reduction end product

Synonym: 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan; Thiazolyl blue formazan

CAS Number: 57360-69-7
Empirical Formula (Hill Notation): C18H17N5S
Molecular Weight: 335.43
MDL Number: MFCD00080731
Linear Formula: C18H17N5S
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-88417-250MG 250 mg
 $56.10
1/EA		 Add To Favorites
45-88417-1G 1 g
 $180.00
1/EA		 Add To Favorites

 

form powder
InChI 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChI key GIFKVQOPAJDJLO-SZVLODPRSA-N
Quality Level 100 
SMILES string Cc1nc(N=NC(=NNc2ccccc2)c3ccccc3)sc1C
solubility chloroform: 10 mg/mL, clear, dark violet
Application: MTT Formazan has been used in cell viability assay.
Biochem/physiol Actions: MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
Other Notes: Reviews: Tetrazolium salts and formazans
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
UNSPSC 12352204

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