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3,3′,5,5′-Tetramethylbenzidine

SIGMA/87748 - ≥98.0% (NT)

Synonym: BM blue; Sure Blue TMB; TMB; TMB Blotting Plus; TMB substrate

CAS Number: 54827-17-7
Empirical Formula (Hill Notation): C16H20N2
Molecular Weight: 240.34
EC Number: 259-364-6
MDL Number: MFCD00007748
Linear Formula: [-C6H2(CH3)2-4-NH2]2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-87748-1G 1 g
 $95.10
1/EA		 Add To Favorites
45-87748-5G 5 g
 $336.00
1/EA		 Add To Favorites
45-87748-25G 25 g
 $1160.00
1/EA		 Add To Favorites
Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 87748-25G

 

assay ≥98.0% (NT)
form powder
InChI 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key UAIUNKRWKOVEES-UHFFFAOYSA-N
mp 166-171 °C
  168-171 °C (lit.)
Quality Level 300 
SMILES string Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
storage temp. 2-8°C
technique(s) UV/Vis spectroscopy: suitable
Application: 3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
Application: 3,3′,5,5′-Tetramethylbenzidine has been used as a substrate for peroxidase in:
• enzyme-immunoassay (EIA)
• indirect competitive enzyme-linked immunosorbent assay (ELISA)
• sandwich enzyme-linked immunosorbent assay
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥98.0% (NT)
mp 166-171 °C; 168-171 °C (lit.)
Storage Temp. 2-8°C
UNSPSC 12352204

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