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5-Carboxyfluorescein

SIGMA/86826 - BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym: 5-FAM

CAS Number: 76823-03-5
Empirical Formula (Hill Notation): C21H12O7
Molecular Weight: 376.32
MDL Number: MFCD00036874
Linear Formula: C21H12O7
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-86826-25MG-F 25 mg
 $59.70
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45-86826-100MG-F 100 mg
 $171.00
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assay ≥95% (HPLC)
fluorescence λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0
form powder
InChI 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
InChI key NJYVEMPWNAYQQN-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
solubility DMF: soluble
  DMSO: soluble
  H2O: soluble
suitability suitable for fluorescence
Application: 5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.
General description: 5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.
Other Notes: Derivative of fluorescein giving stable derivative upon conjugation with biopolymers
Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95% (HPLC)
UNSPSC 12171500

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