HeLa229 cell line
SIGMA/86090201 - NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, from human cervix
Product Type: Chemical
| biological source | human cervix |
| form | liquid |
| growth mode | Adherent |
| karyotype | 2n = 46 |
| morphology | Epithelial |
| packaging | pkg of vial of cells 86090201-1VL |
| tube of 5 μg 86090201-DNA-5UG | |
| products | Not specified |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | cancer |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Tumourigenicity and virus studies, poliovirus 1 and AD3 |
| Biochem/physiol Actions: | Derived from the parent HeLa line but differs chiefly in its relative insusceptibility to polioviruses. The presence of human papilloma virus 18 (HPV-18) sequences in HeLa cells has been reported. |
| Biochem/physiol Actions: | Human cervix carcinoma |
| Biochem/physiol Actions: | STR-PCR Data: Amelogenin: X CSF1PO: 9,10 D13S317: 14 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Disclaimer: | RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization. |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Packaging: | NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. |
| Preparation Note: | EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS). |
| Preparation Note: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41106514 |