SSC Buffer
SIGMA/85639 - for Northern and Southern blotting, powder blend
Synonym: Saline sodium citrate buffer dry blend
Product Type: Chemical
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| form | powder blend |
| grade | Molecular Biology |
| impurities | DNases, none detected |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| product line | BioReagent |
| Quality Level | 100  |
| solubility | H2O: 0.263 g/mL, clear, colorless |
| storage temp. | room temp |
| Application: | Suitable for: • Preparing nucleic acids for hybridization • Hybridization buffer for Northern and Southern blots • Washing buffer for Northern and Southern blots |
| General description: | SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x. |
| Other Notes: | SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl. |
| Preparation Note: | • Convenient pack sizes • Multi-purpose use in hybridizations • Easy-to-use powder makes fast, inexpensive solutions |
| Preparation Note: | Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |