HEPES buffer solution
SIGMA/83264 - 1 M in H2O
Synonym: HEPES solution; N-
CAS Number: 7365-45-9
MDL Number: MFCD00006158
Product Type: Chemical
| application(s) | diagnostic assay manufacturing |
| concentration | 1 M in H2O |
| 238 g/L HEPES in H2O | |
| density | 1.07 g/mL at 20 °C |
| form | liquid |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/C8H18N2O4S/c11-7-5-9-1 |
| InChI key | JKMHFZQWWAIEOD-UHFFFAOYSA |
| pH | 5.5±0.5 |
| Quality Level | 100  |
| SMILES string | OCCN1CCN(CCS(=O)(O)=O)CC1 |
| useful pH range | 6.8-8.2 |
| Application: | HEPES has been used: • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI • As a component of dissection buffer for culturing rat cortical neurons • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses • As a component of Danieu′s aquarium water used in agarose gel preparation |
| General description: | HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.07 g/mL at 20 °C |
| UNSPSC | 12161700 |