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Phenol – chloroform – isoamyl alcohol mixture

SIGMA/77618 - BioUltra, Molecular Biology, 49.5:49.5:1

Synonym: phenol:Chloroform:Isoamyl Alcohol

CAS Number: 136112-00-0
MDL Number: MFCD00133763
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-77618-100ML 100 mL
 $0.00
1/EA
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45-77618-500ML 500 mL
 $368.00
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absorption cut-off at 360 nm
density 1.29 g/mL at 20 °C
form liquid
grade Molecular Biology
impurities DNases, none detected
  phosphatases, none detected
  proteases, none detected
  RNases, none detected
InChI 1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
InChI key ZYWFEOZQIUMEGL-UHFFFAOYSA-N
pH 7.7-8.3(H2O-phase, after extraction with H2O, 1:1)
product line BioUltra
Quality Level 100 
SMILES string ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
storage temp. 2-8°C
Application:

  • Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022 ).

  • Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018 ).

  • The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015 ).

  • The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013 ).
Other Notes: Large- and small-scale phenol extractions
Physical form: mixture of phenol; chloroform; isoamyl alcohol in the ratio of 49.5:49.5:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline
Symbol GHS05GHS06GHS08GHS09  GHS05,GHS06,GHS08,GHS09
Signal word Danger
Hazard statements H301 - H312 + H332 - H314 - H336 - H341 - H351 - H361d - H372 - H373 - H411
Precautionary statements P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Codes T
Risk Statements 63-23/24/25-34-40-48/20/21/22-68
Safety Statements 26-36/37/39-45
RIDADR UN2810 - class 6.1 - PG 2 - EHS - Toxic, liquids, organic, n.o.s
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Density 1.29 g/mL at 20 °C
Storage Temp. 2-8°C
UNSPSC 12352401

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