Tris(2-carboxyethyl)phosphine hydrochloride

SIGMA/75259 - BioUltra, ≥98% (NMR)

Synonym: TCEP

CAS Number: 51805-45-9
Empirical Formula (Hill Notation): C₉H₁₅O₆P · HCl
Molecular Weight: 286.65
MDL Number: MFCD00145469
Linear Formula: C₉H₁₅O₆P · HCl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-75259-1G	1 g	$213.00 1/EA	Add To Favorites
45-75259-10G	10 g	$1080.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

λ	0.1 M in H₂O
anion traces	sulfate (SO₄^2-): ≤50 mg/kg
assay	≥98% (NMR)
cation traces	Al: ≤5 mg/kg
	As: ≤0.05 mg/kg
	Ba: ≤5 mg/kg
	Bi: ≤5 mg/kg
	Ca: ≤10 mg/kg
	Cd: ≤5 mg/kg
	Co: ≤5 mg/kg
	Cr: ≤5 mg/kg
	Cu: ≤5 mg/kg
	Fe: ≤5 mg/kg
	K: ≤50 mg/kg
	Li: ≤5 mg/kg
	Mg: ≤5 mg/kg
	Mn: ≤5 mg/kg
	Mo: ≤5 mg/kg
	Na: ≤50 mg/kg
	Ni: ≤5 mg/kg
	Pb: ≤5 mg/kg
	Sr: ≤5 mg/kg
	Zn: ≤5 mg/kg
form	crystals
InChI	1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
InChI key	PBVAJRFEEOIAGW-UHFFFAOYSA-N
loss	≤1.0% loss on drying
pH	1.2-1.5
product line	BioUltra
Quality Level	300
reaction suitability	reagent type: reductant
SMILES string	Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O
solubility	H₂O: 1 M, clear, colorless (no residue)
storage temp.	2-8°C
UV absorption	λ: 260 nm A_max: 0.05
	λ: 280 nm A_max: 0.03

Application:	Tris(2-carboxyethyl)phosphine hydrochloride has been used: • as a component of IRE1 dialysis buffer, RNA cleavage buffer, and in in vitro cleavage assay of in vitro transcribed RNA • in reducing sulfhydryl groups to obtain total thiol fraction • to reduce protein samples
Application:	Water soluble reagent, used for selective reduction of disulfides. More stable than DTT and useful in mass spectrometry applications.
General description:	Tris(2-carboxyethyl)-phosphine hydrochloride (TCEP) is a strong reducing agent that is stable in an aqueous solution. It readily and stoichiometrically reduces disulfides with high specificity. TCEP exhibits reducing activity at pH 1.5 to pH 9.0 and reduces dehydroascorbic acid (DHA) at pH 4.

Symbol	GHS05
Signal word	Danger
Hazard statements	H314
Precautionary statements	P260 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Codes	C
Risk Statements	34
Safety Statements	26-27-36/37/39
RIDADR	UN 3261 8 / PGII
WGK Germany	WGK 1

Purity	≥98% (NMR)
Storage Temp.	2-8°C
UNSPSC	12352128

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