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4-Nitrophenyl phosphate bis(tris) salt

SIGMA/73737 - for the determination of phosphatase, ≥97.0% (enzymatic)

Synonym: p-Nitrophenylphosphate bis(tris) salt; p-nitrophenyl phosphate bis(tris) salt; PNPP; PNPP bis(tris) salt

CAS Number: 68189-42-4
Empirical Formula (Hill Notation): C14H28N3O12P
Molecular Weight: 461.36
EC Number: 269-198-6
MDL Number: MFCD00037009
Linear Formula: (C6 H4 N O6 P)( C4 H12 N O3)2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-73737-5G 5 g
 $202.00
1/EA		 Add To Favorites
45-73737-25G 25 g
 $801.00
1/EA		 Add To Favorites

 

assay ≥97.0% (enzymatic)
form powder or crystals
impurities ≤0.1% free nitrophenol
  ≤6% water
InChI 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key XXAXKCWOTRABOW-UHFFFAOYSA-N
quality for the determination of phosphatase
Quality Level 100 
SMILES string NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
solubility H2O: 0.1 g/mL, clear
storage temp. 2-8°C
Application: 4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.
Biochem/physiol Actions: 4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate, preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs). Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥97.0% (enzymatic)
Storage Temp. 2-8°C
UNSPSC 12352204

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