Resorufin

SIGMA/73144 - 95%

Synonym: 7-Hydroxy-3H-phenoxazin-3-one; NSC 12097

CAS Number: 635-78-9
Empirical Formula (Hill Notation): C₁₂H₇NO₃
Molecular Weight: 213.19
EC Number: 211-241-8
MDL Number: MFCD00128991
Linear Formula: C₁₂H₇NO₃
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-73144-20MG	20 mg	$163.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥95.0% (HPLC)
	95%
form	solid
InChI	1S/C12H7NO3/c14-7-1-3-9-11(5-7)16-12-6-8(15)2-4-10(12)13-9/h1-6,14H
InChI key	HSSLDCABUXLXKM-UHFFFAOYSA-N
mp	>300 °C (lit.)
Quality Level	100
SMILES string	O=C(C=C1)C=C2C1=NC3=CC=C(O)C=C3O2
storage temp.	−20°C

General description:	Red fluorophore standard. Widely used in enzyme assay systems. Absorption and fluorescence of resorufin are pH dependent. Below the pKa (~6.0), Abs. shifts to ~ 480 nm and both E and fluorescence quantum yield are markedly lower. Resorufin is unstable in the presence of thiols such as dithiothreitol (DTT) and 2-mercaptoethanol.
General description:	Resorufin has high extinction coefficient and good chemical and photostability. These properties of resorufin make it perfect for its usage in inverse fluorescence assay to detect H₂O₂ by measuring the disappearance of its fluorescence upon horseradish peroxidase-mediated oxidation to a nonfluorescent derivative.

Symbol	GHS07
Signal word	Warning
Hazard statements	H315 - H319 - H335
Precautionary statements	P302 + P352 - P305 + P351 + P338
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥95.0% (HPLC); 95%
mp	>300 °C (lit.)
Storage Temp.	−20°C
UNSPSC	12171500

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