Atto 565 NHS ester
SIGMA/72464 - BioReagent, suitable for fluorescence, ≥90% (HPLC)
Empirical Formula (Hill Notation): C35H34ClN3O11
Molecular Weight: 708.11
MDL Number: MFCD03456131
Linear Formula: C35H34ClN3O11
Product Type: Chemical
| assay | ≥90% (degree of coupling) |
| ≥90% (HPLC) | |
| detection method | fluorometric |
| form | powder |
| manufacturer/tradename | ATTO-TEC GmbH |
| product line | BioReagent |
| Quality Level | 100  |
| storage temp. | −20°C |
| suitability | suitable for fluorescence |
| Features and Benefits: | • Strong absorption • High fluorescence quantum yield • High thermal and photo-stability |
| General description: | Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling. |
| Legal Information: | This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing. |
| Other Notes: | Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (HPLC); ≥90% (degree of coupling) |
| Storage Temp. | −20°C |
| UNSPSC | 12352108 |