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4-Nitrophenyl phosphate disodium salt hexahydrate

SIGMA/71768 - suitable for enzyme immunoassay, ≥99.0% (enzymatic)

Synonym: p-Nitrophenyl phosphate disodium hexahydrate; Disodium 4-nitrophenyl phosphate hexahydrate; di-Sodium 4-nitrophenyl phosphate; pNPP disodium hexahydrate

CAS Number: 333338-18-4
Empirical Formula (Hill Notation): C6H4NNa2O6P · 6H2O
Molecular Weight: 371.14
EC Number: 224-246-5
MDL Number: MFCD00066288
Linear Formula: O2NC6H4OP(O)(ONa)2 · 6H2O
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-71768-5G 5 g
 $147.00
1/EA		 Add To Favorites
45-71768-25G 25 g
 $583.00
1/EA		 Add To Favorites
pNPP substrates develop the yellow end-product, p-nitrophenol, when hydrolyzed by alkaline phosphatase. Since this system forms a soluble end product, it is not recommended for blotting or histochemistry.

 

assay ≥99.0% (enzymatic)
form powder or crystals
impurities ≤0.1% free nitrophenol
InChI 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
InChI key KAKKHKRHCKCAGH-UHFFFAOYSA-L
Quality Level 100 
SMILES string O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
storage temp. 2-8°C
technique(s) enzyme immunoassay: suitable
Application: 4-nitrophenyl phosphate disodium salt hexahydrate has been used:
• as a substrate to measure the kinetic parameters of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphatase activity
• as substrate to assay acid phosphatase activity
• as substrate to assay bound antibodies with anti-rabbit/human alkaline phosphatase conjugate
General description: Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥99.0% (enzymatic)
Storage Temp. 2-8°C
UNSPSC 12352204

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