Sodium acetate
SIGMA/71183 - anhydrous, BioUltra, suitable for luminescence, Molecular Biology, ≥99.0% (NT)
Synonym: Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous; Acetic acid sodium salt
CAS Number: 127-09-3
Empirical Formula (Hill Notation): C2H3NaO2
Molecular Weight: 82.03
EC Number: 204-823-8
MDL Number: MFCD00012459
Linear Formula: CH3COONa
Product Type: Chemical
| λ | 3 M in H2O |
| anion traces | chloride (Cl-): ≤20 mg/kg |
| phosphate (PO43-): ≤5 mg/kg | |
| sulfate (SO42-): ≤20 mg/kg | |
| assay | ≥99.0% (NT) |
| autoignition temp. | 1112 °F |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤10 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤200 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤10 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | solid |
| grade | Molecular Biology |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/C2H4O2.Na/c1-2(3)4;/h1 |
| InChI key | VMHLLURERBWHNL-UHFFFAOYSA |
| loss | ≤0.5% loss on drying, 150 °C |
| mp | >300 °C (dec.) (lit.) |
| pH | 8.5-10.0 (25 °C, 3 M in H2O) |
| pKa | 4.76 (acetic acid) |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | [Na+].CC([O-])=O |
| solubility | H2O: 3 M at 20 °C, clear, colorless |
| suitability | suitable for luminescence |
| UV absorption | λ: 260 nm Amax: 0.03 |
| λ: 280 nm Amax: 0.02 |
| Application: | Sodium acetate is suitable for use as a buffer in:• protein crystallization • staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography • the preparation of standard (acetylcholine chloride) for hestrin assay • substrate screening characterization for microfluidic microbial fuel cell array |
| Biochem/physiol Actions: | Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather. |
| Other Notes: | Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥99.0% (NT) |
| mp | >300 °C (dec.) (lit.) |
| UNSPSC | 12161700 |