Methyl Green

SIGMA/67060 - zinc chloride salt, suitable for microscopy

Synonym: Ethyl Green

CAS Number: 7114-03-6
Empirical Formula (Hill Notation): C₂₇H₃₅BrClN₃ · ZnCl₂
Molecular Weight: 653.24
EC Number: 230-415-4
MDL Number: MFCD00151094
Linear Formula: C₂₇H₃₅BrClN₃ · ZnCl₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-67060-10G	10 g	$167.00 1/EA		Add To Favorites
45-67060-50G	50 g	$852.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

ε_max	≥900 at 624-634 nm in water
application(s)	diagnostic assay manufacturing hematology histology
form	powder
InChI	1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
InChI key	RRKSQWOQZFIQKW-UHFFFAOYSA-J
mp	>300 °C (lit.)
pH	0.2-1.8, yellow to blue
Quality Level	200
SMILES string	[Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)C(c2ccc(cc2)N(C)C)=C3/C=CC(C=C3)=[N+](C)C
solubility	water: 0.01 g/10 mL, blue to very deep blue
storage temp.	room temp
suitability	suitable for microscopy
technique(s)	titration: suitable

Analysis Note:	Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts.
Application:	Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.
Biochem/physiol Actions:	Marker dye for DNA in acid and neutral media. Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

Symbol	GHS05,GHS07,GHS09
Signal word	Danger
Hazard statements	H314 - H335 - H411
Precautionary statements	P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

mp	>300 °C (lit.)
Storage Temp.	room temp
Colour Index Number	42590
UNSPSC	12171500

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