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DL-Dithiothreitol solution

SIGMA/646563 - 1 M in H2O

Synonym: (±)-threo-1,4-Dimercapto-2,3-butanediol solution; DTT

CAS Number: 3483-12-3
Empirical Formula (Hill Notation): C4H10O2S2
Molecular Weight: 154.25
MDL Number: MFCD00004877
Linear Formula: C4H10O2S2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-646563-10X.5ML 0.5 mL
 $113.00
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concentration 1 M in H2O
density 1.035 g/mL at 25 °C
  1.04 g/mL at 20 °C
form liquid
InChI 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key VHJLVAABSRFDPM-QWWZWVQMSA-N
Quality Level 100 
reaction suitability reagent type: reductant
SMILES string O[C@H](CS)[C@H](O)CS
Application: DL-Dithiothreitol solution has been used:
• in western blot analysis
• as a supplement in lysis buffer to study its effects on sperm lysis
• as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
• in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds
Features and Benefits: Low toxicity and reduced odor compared to other thiol compounds.
General description: Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
General description: Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.
Packaging: 10×0.5 mL in ampule
Density 1.04 g/mL at 20 °C; 1.035 g/mL at 25 °C
UNSPSC 12161504

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