3-Methylbutanol
SIGMA/59085 - BioUltra, Molecular Biology, ≥99.0% (GC)
Synonym: 3-Methyl-1-butanol; Isoamyl alcohol; Isopentyl alcohol
CAS Number: 123-51-3
Empirical Formula (Hill Notation): C5H12O
Molecular Weight: 88.15
EC Number: 204-633-5
MDL Number: MFCD00002934
Linear Formula: (CH3)2CHCH2CH2OH
Product Type: Chemical
| λ | neat |
| assay | ≥99.0% (GC) |
| cation traces | Al: ≤0.5 mg/kg |
| Ba: ≤0.1 mg/kg | |
| Bi: ≤0.1 mg/kg | |
| Ca: ≤0.5 mg/kg | |
| Cd: ≤0.05 mg/kg | |
| Co: ≤0.02 mg/kg | |
| Cr: ≤0.02 mg/kg | |
| Cu: ≤0.02 mg/kg | |
| Fe: ≤0.1 mg/kg | |
| K: ≤0.5 mg/kg | |
| Li: ≤0.1 mg/kg | |
| Mg: ≤0.1 mg/kg | |
| Mn: ≤0.02 mg/kg | |
| Mo: ≤0.1 mg/kg | |
| Na: ≤0.5 mg/kg | |
| Ni: ≤0.02 mg/kg | |
| Pb: ≤0.1 mg/kg | |
| Sr: ≤0.1 mg/kg | |
| Zn: ≤0.1 mg/kg | |
| density | 0.809 g/mL at 20 °C (lit.) |
| evapn. residue | ≤0.003% |
| form | liquid |
| grade | Molecular Biology |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| ≤0.01% free acid (as CH3COOH) | |
| InChI | 1S/C5H12O/c1-5(2)3-4-6/h5 |
| InChI key | PHTQWCKDNZKARW-UHFFFAOYSA |
| pH | 5.6 (20 °C, 25 g/L) |
| product line | BioUltra |
| Quality Level | 100  |
| refractive index | n |
| SMILES string | CC(C)CCO |
| technique(s) | DNA extraction: suitable |
| RNA extraction: suitable | |
| UV absorption | λ: 260 nm Amax: 0.06 |
| λ: 280 nm Amax: 0.06 |
| Application: | Isoamyl alcohol (3-methylbutanol) may be used as a protein denaturation component of various RNA and DNA extraction and purification protocols. Isoamyl alcohol is frequently used together with phenol and or chloroform in nucleic acid preparation protocols. |
| Other Notes: | Reduces interface between 2 phases |
| Symbol |    GHS02,GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H226 - H315 - H318 - H332 - H335 |
| Precautionary statements | P210 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 + P310 |
| Hazard Codes | Xn |
| Risk Statements | 10-20-36/37/38-66 |
| Safety Statements | 26-46 |
| RIDADR | UN1105 - class 3 - PG 3 - Pentanols |
| WGK Germany | WGK 1 |
| Flash Point(F) | 109.4 °F - closed cup |
| Flash Point(C) | 43 °C - closed cup |
| Purity | ≥99.0% (GC) |
| Density | 0.809 g/mL at 20 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12352104 |