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Iodonitrotetrazolium chloride

SIGMA/58030 - BioReagent, ≥97.0% (calc. on dry substance, NT)

Synonym: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; p-Iodonitrotetrazolium Violet; INT

CAS Number: 146-68-9
Empirical Formula (Hill Notation): C19H13ClIN5O2
Molecular Weight: 505.70
EC Number: 205-676-2
MDL Number: MFCD00011961
Linear Formula: C19H13ClIN5O2
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-58030-1G-F 1 g
 $0.00
1/EA
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45-58030-5G-F 5 g
 $0.00
1/EA
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45-58030-25G-F 25 g
 $2630.00
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application(s) histology
assay ≥97.0% (calc. on dry substance, NT)
InChI 1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1
InChI key JORABGDXCIBAFL-UHFFFAOYSA-M
mp 240 °C (dec.) (lit.)
  ~245 °C (dec.)
product line BioReagent
Quality Level 200 
SMILES string [Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4
solubility methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)
Application: Electron acceptor for the colorimetric assay of various dehydrogenases
Application: Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.
Purity ≥97.0% (calc. on dry substance, NT)
mp 240 °C (dec.) (lit.); ~245 °C (dec.)
UNSPSC 12352102

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