Alcohol Dehydrogenase equine
SIGMA/55689 - recombinant, expressed in E. coli, ≥0.5 U/mg
Synonym: ADH
CAS Number: 9031-72-5
EC Number: 232-870-4
MDL Number: MFCD00081305
Product Type: Chemical
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| application(s) | life science and biopharma |
| biological source | equine |
| color | white |
| light yellow | |
| description | Isozyme E sequence |
| form | lyophilized powder |
| pH | 7 |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| solubility | water: 5 mg/mL |
| specific activity | ≥0.5 U/mg |
| storage temp. | −20°C |
| Application: | Alcohol Dehydrogenase equine has been used in in vitro alcohol dehydrogenase (Adh) assay. |
| Biochem/physiol Actions: | Alcohol dehydrogenase catalyzes the oxidative conversion of alcohol into aldehyde. It has a homodimeric structure with a co-enzyme binding domain at the C-terminal and an N-terminal catalytic domain. The active site is located at the interdomain cleft. Binding of NAD+ in the active site causes conformational changes which create the binding site for the alcohol substrate. |
| Biochem/physiol Actions: | Horse liver alcohol dehydrogenase (HL-ADH) is an enzyme with broad specificity, capable of catalyzing the reversible oxidation of a wide variety of primary and secondary alcohols to form their corresponding aldehydes and ketones. Moreover, alcohol dehydrogenase can oxidize ethanol while simultaneously reducing nicotinamide adenine dinucleotide (NAD+) to NADH. Previous studies have demonstrated that ADH and ALDH variants can influence alcohol dependence. Additionally, the ADH genotype has been linked to lacunar infarction and neuropsychiatric diseases. |
| General description: | Research Area: Neuroscience Alcohol dehydrogenase is a zinc metalloprotein that forms five classes of isoenzymes through the dimerization of eight different subunits. |
| Other Notes: | 1 U corresponds to the amount of enzyme which reduces 1 μmol benzaldehyde per minute at pH 7.0 and 30 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.5 U/mg |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.1.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |