Lipase B Candida antarctica immobilized on Immobead 150, recombinant from yeast
SIGMA/52583 - ≥2000 U/g
Synonym: Candida antarctica Lipase
Product Type: Chemical
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| form | beads |
| mol wt | 33 kDa |
| Quality Level | 100  |
| recombinant | expressed in yeast |
| specific activity | ≥2000 U/g |
| storage temp. | 2-8°C |
| Application: | Lipase B Candida antarctica immobilized on Immobead 150, recombinant from yeast has been used: • in esterification reaction of lauric acid with n-butanol in a biphasic solvent system • in hydrolysis of fish oil triglycerides • in screening of enzymes for Morita–Baylis–Hillman (MBH) reaction |
| Application: | Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel. |
| Biochem/physiol Actions: | Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses. |
| Biochem/physiol Actions: | Lipases B from Candida antarctica (CALB) is a versatile catalyst for biotransformation reactions. In aqueous media, CALB behaves like an esterase rather than a lipase. |
| Biochem/physiol Actions: | Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids. |
| General description: | Lipase B or triacylglycerol ester hydrolases belongs to the class of hydrolases. Lipase B from Candida antarctica (CALB) possesses 317 amino acids and a molecular weight of 33 kDa. CALB has a catalytic triad and an open solvent accessible active site. The catalytic triad is usually Ser-His-Asp/Glu which is found in the carboxy terminal of parallel β sheet. |
| Other Notes: | 1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2000 U/g |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |