RPMI-1640 Medium
SIGMA/51536C - with 2.05 mM L-glutamine, with 25mM HEPES, liquid, sterile-filtered, suitable for cell culture
MDL Number: MFCD00217820
Product Type: Chemical
| components | HEPES: 5958 mg/L L-glutamine: 300 mg/L NaHCO3: 2000 mg/L phenol red: 5.310 mg/L |
| description | for research or for further manufacturing use |
| form | liquid |
| Quality Level | 500  |
| shipped in | ambient |
| sterility | sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | hybridoma: suitable |
| cell culture | mammalian: suitable |
| Application: | RPMI-1640 Medium has been used to incubate dissected lung tissue. |
| General description: | RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |