TRIS borate-EDTA buffer concentrate, long run
SIGMA/51309 - for Northern and Southern blotting, solution
MDL Number: MFCD00217350
Product Type: Chemical
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| form | solution |
| grade | Molecular Biology |
| impurities | DNases, none detected |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| product line | BioReagent |
| quality | 10× concentrate |
| ready-to-use solution | |
| Quality Level | 100  |
| storage temp. | room temp |
| Application: | Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. |
| General description: | TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water). |
| Preparation Note: | The TBE 10x concentrate contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H360FD |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | T |
| Risk Statements | 60-61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |