PureCol™
SIGMA/5006 - Collagen powder from Bovine hide, 97% Type I with the remainder of Type III collagen, suitable for cell culture
Synonym: Collagen Type I; PureCol Collagen
EC Number: 232-697-4
Product Type: Product-on-demand
| assay | 99.9% |
| binding specificity | Peptide Source: Elastin |
| Peptide Source: Fibrinogen |
|
| biological source | bovine |
| form | lyophilized |
| packaging | pkg of 15 mg |
| Quality Level | 100  |
| shipped in | wet ice |
| sterility | sterile-filtered |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | PureCol™ has been used: • in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids • to coat Costar® Transwell® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC) • to coat culture plates for collagen degradation assay with breast cancer cell lines |
| General description: | PureCol™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography. It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol™, lyophilized form is not recommended for the formation of a solid gel. PureCol™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder. |
| Legal Information: | Costar is a registered trademark of Corning, Inc. |
| Legal Information: | PureCol is a trademark of Advanced BioMatrix, Inc. |
| Legal Information: | Transwell is a registered trademark of Corning, Inc. |
| Preparation Note: | This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 99.9% |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |