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Fura 2-AM

SIGMA/47989 - BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

Synonym: Fura-2 pentakis(acetoxymethyl) ester

CAS Number: 108964-32-5
Empirical Formula (Hill Notation): C44H47N3O24
Molecular Weight: 1001.85
MDL Number: MFCD00036976
Linear Formula: C44H47N3O24
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-47989-1MG-F 1 mg
 $578.00
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assay ≥95.0% (HPLC)
fluorescence λex 355 nm; λem 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca2+
form solid
InChI 1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3
InChI key VPSRLGDRGCKUTK-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O
solubility DMSO: soluble
storage temp. −20°C
suitability suitable for fluorescence
Application: Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells.
Features and Benefits: The major benefits of using the ester form of Fura 2 as Fura 2-AM are:

•  It is cell-permeable.
• It can be loaded into cells easily.
Other advantages of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of :
• The dye concentration.
• Illumination intensity, and
• Optical path length.
General description: Fura 2-AM (Fura 2), also known as acetoxy-methyl-ester, is a calcium-specific ratio-metric fluorescent dye. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505nm, and the excitation peak ranges between 340-380nm and is the preferred calcium indicator.
Other Notes: Membrane-permeable derivative of Fura-2. Used to load the chelator into cells. Hydrolysed enzymatically in the cell to yield Fura-2 which remains within the cell
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95.0% (HPLC)
Storage Temp. −20°C
UNSPSC 12171500

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