Phosphohistone H3 (PHH3) Rabbit Polyclonal Antibody
SIGMA/369A-1
Product Type: Chemical
Immunohistochemistry Phosphohistone H3 (PHH3) (polyclonal) on melanoma tissue
| antibody form | Ig fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| control | tonsil |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| manufacturer/tradename | Cell Marque® |
| packaging | bottle of 1.0 mL predilute (369A-17) |
| bottle of 7.0 mL predilute (369A-18) | |
| vial of 0.1 mL concentrate (369A-14) | |
| vial of 0.5 mL concentrate (369A-15) | |
| vial of 1.0 mL concentrate (369A-16) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500 |
| visualization | nuclear |
| Analysis Note: |
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| General description: | Phosphohistone H3 (PHH3) is a core histone protein, which together with other histones, forms the major protein constituents of the chromatin in eukaryotic cells. In mammalian cells, phosphohistone H3 is negligible during interphase but reaches a maximum for chromatin condensation during mitosis. Immunohistochemical studies showed anti-PHH3 specifically detected the core protein histone H3 only when phosphorylated at serine 10 or serine 28. Studies have also revealed no phosphorylation on the histone H3 during apoptosis. Therefore, PHH3 can serve as a mitotic marker to separate mitotic figures from apoptotic bodies and karyorrhectic debris, which may be a very useful tool in the diagnosis of tumor grades, especially in CNS, skin, gyn., soft tissue, and GIST. | ||||
| Legal Information: | Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany | ||||
| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com | ||||
| Other Notes: | Phosphohistone H3 (PHH3) Positive Control Slides, Product No. 369S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
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| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide | ||||
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |