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5(6)-Carboxyfluorescein diacetate

SIGMA/21879 - BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Synonym: 5(6)-Carboxy-di-O-acetylfluorescein

CAS Number: 124387-19-5
Empirical Formula (Hill Notation): C25H16O9
Molecular Weight: 460.39
MDL Number: MFCD00467494
Linear Formula: C25H16O9
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-21879-25MG-F 25 mg
 $88.40
1/EA		 Add To Favorites
45-21879-100MG-F 100 mg
 $295.00
1/EA		 Add To Favorites

 

assay ≥90.0% (HPLC)
fluorescence λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)
form powder
InChI 1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)
InChI key SWRGCNMDGRMQGB-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1.CC(=O)Oc6ccc7c(Oc8cc(OC(C)=O)ccc8C79OC(=O)c%10cccc(C(O)=O)c9%10)c6
solubility DMSO: soluble
storage temp. 2-8°C
suitability suitable for fluorescence
Application: 5(6)-Carboxyfluorescein diacetate (CFDA) is a dye which is been extensively utilized to label cultured neurons with the essential requirements, in a specific manner.

5(6)- Carboxyfluorescein diacetate (CFDA) is a viability probe that assesses enzymatic activity, which is necessary to activate its fluorescence, and cell-membrane integrity, which is necessary for the retention of their fluorescent product within the cell.
General description: 5(6)-Carboxyfluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥90.0% (HPLC)
Storage Temp. 2-8°C
UNSPSC 12352108

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