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5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

SIGMA/21878 - BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Synonym: 5(6)-FAM SE; FLUOS

CAS Number: 117548-22-8
Empirical Formula (Hill Notation): C25H15NO9
Molecular Weight: 473.39
MDL Number: MFCD04119713
Linear Formula: C25H15NO9
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-21878-25MG-F 25 mg
$82.80
1/EA
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45-21878-100MG-F 100 mg
$251.00
1/EA
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assay ≥80% (HPLC)
fluorescence λex 492 nm; λem 517 nm in DMF
form powder
InChI 1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)
InChI key YVTPVJDECOUTJW-UHFFFAOYSA-N
product line BioReagent
Quality Level 200 
SMILES string OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10
solubility DMF: soluble
  DMSO: soluble
storage temp. −20°C
suitability suitable for fluorescence
Application: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.
Application: Fluorescent label for primary and secondary amine groups on proteins and other biomolecules.
Application: Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA)
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥80% (HPLC)
Storage Temp. −20°C
UNSPSC 12352108

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