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5(6)-Carboxyfluorescein

SIGMA/21877 - BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym: 5(6)-FAM; 5,6-CF

CAS Number: 72088-94-9
Empirical Formula (Hill Notation): C21H12O7
Molecular Weight: 376.32
EC Number: 276-331-1
MDL Number: MFCD00151081
Linear Formula: C21H12O7
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-21877-1G-F 1 g
 $63.10
1/EA		 Add To Favorites
45-21877-5G-F 5 g
 $214.00
1/EA		 Add To Favorites

 

assay ≥95% (HPLC)
fluorescence λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0
form powder
InChI 1S/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)
InChI key BPVHBBXCESDRKW-UHFFFAOYSA-N
mp 275 °C (dec.) (lit.)
product line BioReagent
Quality Level 200 
SMILES string OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35.OC(=O)c6ccc7C(=O)OC8(c9ccc(O)cc9Oc%10cc(O)ccc8%10)c7c6
suitability suitable for fluorescence
Application: 5(6)-Carboxyfluorescein is used to characterize tumor cells and normal cells in vivo. It labels proteins derived through N-hydroxysuccinimide (NHS) ester-mediated derivatization. 5(6)-Carboxyfluorescein is suitable for detecting cell viability while developing an in vitro anthelmintic drug assay.
General description: 5(6)-Carboxyfluorescein, also known as 5(6)-FAM, is a fluorescent tracer and available as a mixture of 5-Carboxyfluorescein and 6-Carboxyfluorescein isomers. The fluorescence of 5(6)-Carboxyfluorescein is determined by the xanthene moiety. 5(6)-Carboxyfluorescein has two characteristic properties: • It has 2 wavelengths for maximum absorbance (465 and 490nm).
• And its fluorescence emission, at 515nm(max.), increases as a function of pH in the physiological range of 6-7.4.
Other Notes: A probe of intracellular pH
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95% (HPLC)
mp 275 °C (dec.) (lit.)
UNSPSC 12171500

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