5(6)-Carboxyfluorescein
SIGMA/21877 - BioReagent, suitable for fluorescence, ≥95% (HPLC)
Synonym: 5(6)-FAM; 5,6-CF
CAS Number: 72088-94-9
Empirical Formula (Hill Notation): C21H12O7
Molecular Weight: 376.32
EC Number: 276-331-1
MDL Number: MFCD00151081
Linear Formula: C21H12O7
Product Type: Chemical
assay | ≥95% (HPLC) |
fluorescence | λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 |
form | powder |
InChI | 1S/2C21H12O7/c22-11-2-5-1 |
InChI key | BPVHBBXCESDRKW-UHFFFAOYSA |
mp | 275 °C (dec.) (lit.) |
product line | BioReagent |
Quality Level | 200 |
SMILES string | OC(=O)c1ccc2c(c1)C(=O)OC2 |
suitability | suitable for fluorescence |
Application: | 5(6)-Carboxyfluorescein is used to characterize tumor cells and normal cells in vivo. It labels proteins derived through N-hydroxysuccinimide (NHS) ester-mediated derivatization. 5(6)-Carboxyfluorescein is suitable for detecting cell viability while developing an in vitro anthelmintic drug assay. |
General description: | 5(6)-Carboxyfluorescein, also known as 5(6)-FAM, is a fluorescent tracer and available as a mixture of 5-Carboxyfluorescein and 6-Carboxyfluorescein isomers. The fluorescence of 5(6)-Carboxyfluorescein is determined by the xanthene moiety. 5(6)-Carboxyfluorescein has two characteristic properties: • It has 2 wavelengths for maximum absorbance (465 and 490nm). • And its fluorescence emission, at 515nm(max.), increases as a function of pH in the physiological range of 6-7.4. |
Other Notes: | A probe of intracellular pH |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥95% (HPLC) |
mp | 275 °C (dec.) (lit.) |
UNSPSC | 12352200 |