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Calcein-AM

SIGMA/17783 - BioReagent, suitable for fluorescence, ≥95.0% (HPLC)

Synonym: Calcein acetoxymethyl ester; Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester

CAS Number: 148504-34-1
Empirical Formula (Hill Notation): C46H46N2O23
Molecular Weight: 994.86
MDL Number: MFCD05861516
Linear Formula: C46H46N2O23
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-17783-1MG 1 mg
 $568.00
1/EA		 Add To Favorites
45-17783-5MG 5 mg
 $2460.00
1/EA		 Add To Favorites

 

assay ≥95.0% (HPLC)
form solid
InChI 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
InChI key XKFSBWQWNMZWFA-UHFFFAOYSA-N
product line BioReagent
Quality Level 100 
SMILES string CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
storage temp. −20°C
suitability suitable for fluorescence
Application: Calcein-AM has been used in flow cytometry.
Application: Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.
General description: Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥95.0% (HPLC)
Storage Temp. −20°C
UNSPSC 12352200

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