Bromocresol Purple
SIGMA/17490 - for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)
Synonym: 5,5′-Dibromo-o-cresolsulfonphthalein; Bromcresol purple sultone form
CAS Number: 115-40-2
Empirical Formula (Hill Notation): C21H16Br2O5S
Molecular Weight: 540.22
EC Number: 204-087-8
MDL Number: MFCD00011681
Linear Formula: C21H16Br2O5S
Product Type: Chemical
| εmax | ≥400 at 414-424 nm in methanol |
| application(s) | diagnostic assay manufacturing hematology histology |
| form | powder |
| grade | for microscopy (Hist., Vit.) |
| indicator (pH 5.2-6.8) | |
| InChI | 1S/C21H16Br2O5S/c1-11-7-1 |
| InChI key | ABIUHPWEYMSGSR-UHFFFAOYSA |
| mp | 240 °C (dec.) (lit.) |
| pH | 5.2-6.8, yellow to purple |
| Quality Level | 200  |
| SMILES string | Cc1cc(cc(Br)c1O)C2(OS(=O) |
| solubility | hot ethanol: 0.1 g/10 mL |
| storage temp. | room temp |
| technique(s) | titration: suitable |
| Application: | Spectrophotometric determination of serum-albumin |
| General description: | Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 240 °C (dec.) (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |