CD71 (MRQ-48) Mouse Monoclonal Antibody
SIGMA/171M-9
Product Type: Chemical
Immunohistochemistry CD71 (MRQ-48) on bone marrow
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | MRQ-48, monoclonal |
| conjugate | unconjugated |
| control | bone marrow |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| isotype | IgG1 |
| manufacturer/tradename | Cell Marque™ |
| packaging | bottle of 1.0 mL predilute (171M-97) |
| bottle of 7.0 mL predilute (171M-98) | |
| vial of 0.1 mL concentrate (171M-94) | |
| vial of 0.5 mL concentrate (171M-95) | |
| vial of 1.0 mL concentrate (171M-96) | |
| Quality Level | 100  |
| 500 |
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| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500 |
| visualization | cytoplasmic, membranous |
| General description: | The transferrin receptor (CD71) is most highly expressed on placental syncytiotrophoblasts, myocytes, basal keratinocytes, hepatocytes, endocrine pancreas, spermatocytes, and erythroid precursors. The level of transferrin receptor expression is highest in early erythroid precursors through the intermediate normoblast phase, after which expression decreases through the reticulocyte phase. The maturation of erythrocytes results in loss of transferrin receptor expression, in concert with down-regulation of the machinery for hemoglobin synthesis. The high level of transferrin receptor within erythroid precursors makes it an excellent marker for evaluation of erythroid components within bone marrow. Anti-CD71 is a highly effective marker for highlighting erythroid precursors in bone marrow biopsy specimens and shows the following features: 1) distinct membranous and cytoplasmic staining pattern, which is easily recognized in bone marrow biopsy; 2) restriction to erythroid lineage within bone marrow biopsy specimens; 3) CD71 expression decreases with the maturation of erythrocytes, with the highest level seen in early forms and the lowest level in late normoblast stage, and most importantly; 4) mature erythrocytes do not express CD71, which facilitates bone marrow analyses.Anti-CD71 is useful in identifying erythroid precursors with very little interference from mature erythrocytes and also in the determination of erythroid leukemia, benign erythroid proliferative disorders, and myelodysplastic syndrome, although further studies are needed for making a definitive diagnosis of MDS. | ||||
| Legal Information: | Cell Marque is a trademark of Sigma-Aldrich Co. LLC | ||||
| Linkage: | CD71 Positive Control Slides, Product No. 171S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
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| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com | ||||
| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide | ||||
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. |
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| Quality: |
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| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |