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EX-CELL® 420 Serum-Free Medium for Insect Cells

SIGMA/14420C - with L-glutamine, sterile-filtered, suitable for insect cell culture

MDL Number: MFCD01634638
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-14420C-500ML 500 mL
 $74.00
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45-14420C-1000ML 1000 mL
 $135.00
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components HEPES: no
L-glutamine: yes
glucose: yes
NaHCO3: yes
description for research or for further manufacturing use
form liquid
product line EX-CELL®
Quality Level 500 
shipped in ambient
sterility sterile-filtered
storage temp. 2-8°C
technique(s) cell culture | insect: suitable
Application: EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
Application: EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
Legal Information: EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352207

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