CD35 (EP197) Rabbit Monoclonal Primary Antibody
SIGMA/135R-1
Synonym: C3-binding protein; C3BR; C3b/C4b receptor; C4BR; CD35 antigen; CR1; Complement Receptor 1; Complement component (3b/4b) receptor 1 (Knops blood group); Knops blood group antigen
Product Type: Chemical
Immunohistochemistry CD35 (EP197) on follicular dendritic cell sarcoma
| antibody form | culture supernatant |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | EP197, monoclonal |
| concentration | 0.1-5.0 μg/mL (predilute) |
| 2.5-125 μg/mL (concentrate) | |
| conjugate | unconjugated |
| control | tonsil |
| description | For In Vitro Diagnostic Use in Select Regions (See Chart) |
| form | buffered aqueous solution |
| isotype | IgG |
| manufacturer/tradename | Cell Marque™ |
| packaging | vial of 0.1 mL concentrate (135R-14) |
| vial of 0.1 mL concentrate Research Use Only (135R-14-RUO) | |
| vial of 0.5 mL concentrate (135R-15) | |
| vial of 1.0 mL concentrate (135R-16) | |
| vial of 1.0 mL concentrate Research Use Only (135R-16-RUO) | |
| vial of 1.0 mL pre-dilute Research Use Only (135R-17-RUO) | |
| vial of 1.0 mL predilute ready-to-use (135R-17) | |
| vial of 7.0 mL pre-dilute ready-to-use Research Use Only (135R-18-RUO) | |
| vial of 7.0 mL predilute ready-to-use (135R-18) | |
| Quality Level | 100  |
| 500 |
|
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100 (concentrated) |
| visualization | membranous |
| General description: | CD35 (complement receptor 1) is a cell membrane-bound, monomeric glycoprotein found on numerous cell types including erythrocytes, leukocytes, glomerular podocytes, and follicular dendritic cells. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. CD35 antigen is found on erythrocytes, B cells, a subset of T cells, monocytes, as well as eosinophils and neutrophils. Anti-CD35 is considered a mature B-cell marker which labels follicular dendritic reticulum cells and tumors derived from such cells such as follicular dendritic cell tumor/sarcoma. |
| Legal Information: | Cell Marque is a trademark of Sigma-Aldrich Co. LLC |
| Linkage: | CD35 Positive Control Slides, Product No. 135S , are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections). |
| Other Notes: | For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com |
| Physical form: | Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide |
| Preparation Note: | Download the IFU specific to your product lot and format  Note: This requires a keycode which can be found on your packaging or product label. Download the latest released IFU Note: This IFU may not apply to your specific product lot. |
| Quality: | United States - IVD Canada - IVD European Union - IVD Japan - RUO |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |