CHO-K1-AC-free
SIGMA/13080801
Product Type: Chemical
| biological source | hamster ovary |
| description | Hamster Chinese Ovary, serum-free |
| growth mode | suspension |
| morphology | Aggregates in suspension |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Cell Line Description: | This is a derviative of the CHO-K1 cell line, ECACC catalogue no. 85051005 which has been adapted to grow in the animal component free medium, CHO PF Media (C5467) |
| Culture Medium: | CHO PF Media (C5467) + 8mM L-Glutamine (G7513) . When freezing cells use freeze medium composed of 50:50 fresh:conditioned medium + 10% DMSO. |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Subculture Routine: | Maintain cultures between approximately 3-9 x 105 cells/ml; 5% CO2; 37°C. Cells grow as aggregates in suspension. Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |