Rat Tail Collagen Coating Solution (20 ML)
SIGMA/122-20
Product Type: Product-on-demand
| form | liquid |
| packaging | pkg of 20 mL |
| Quality Level | 100  |
| shelf life | 6 mo. |
| shipped in | wet ice |
| sterility | sterile; sterile-filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P02454  |
| Application: | Coat tissue culture ware with Rat Tail Collagen Solution to culture surface ratio of 5 ug/cm2. For example: 125 ug (2.5 ml) for a T-25 flask. 10.5 ug (210 ul) for each well of a 24-well plate. Coat tissue culture ware at 37oC for 2 hours. Carefully aspirate Collagen Coating Solution. Rinse the coated surface twice with PBS. Coated tissue culture ware may be used immediately or air-dried and stored at 4 oC for up to one week. |
| General description: | Rat Tail Collagen Coating Solution (20 ML) enhances cell attachment and proliferation, or promotion of cell-specific morphology and function. It is commonly used for endothelial cells, hepatocytes, muscle cells, and a variety of other cell types. This collagen solution is prepared in 0.02 M Acetic Acid at 50 ug/ml and sterile filtered. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |