L5178Y tk+/- 3.7.2c (IVGT)
SIGMA/12080201 - from mouse thymus
Product Type: Product-on-demand
| biological source | mouse thymus |
| description | Mouse DBA/2 lymphoma L5178Y tk+/- |
| growth mode | Suspension |
| morphology | Small rounded cells |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Cell Line Description: | The L5178Y tk+/- 3.7.2c (IVGT) mouse lymphoma cell line was derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene treatment. The cell line is tumourigenic in DBA/2 mice. The cell line can be used in genotoxicity tests including gene mutation assays at the tk and other loci and micronucleus induction. |
| Culture Medium: | Fischer′s medium + 2mM Glutamine + 1% Sodium pyruvate + 10% heat-inactivated Horse Serum (HS) |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Other Notes: | Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply  for more information. |
| Subculture Routine: | Maintain cultures in logarithmic growth phase between 3 x 104 and 1 x 106 cells/ml. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |