SK-GT-2 cell line
SIGMA/11012008
Product Type: Chemical
| biological source | human esophagus |
| growth mode | Adherent |
| karyotype | Modal number 58 chromosomal aberration at 11p13-15. |
| morphology | Epithelial |
| products | Expression of actin imtermediate filaments and growth factors TGF?1, TGF?2, TGF?3, TGF? and platelet-derived growth factor A (PDGFA). |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | metastasis |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Cell Line Description: | SK-GT-2 was established from a primary adenocarcinoma of gastric fundus from a 72-year-old Hispanic male previously treated for squamous cell carcinoma with radiation therapy and radical neck dissection. Pathology of recurrence (1 year) demonstrated ulcerating poorly differentiated adenocarcinoma of the stomach invading spleen and epigastic fat (T4) with metastases to perisplenic and perigastric nodes (N1). SK-GT-2 was found to be tumorigenic in athymic nu/nu mice. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. |
| Cell Line Origin: | Adenocarcinoma of the Gastric fundus, poorly differentiated |
| Culture Medium: | RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) |
| DNA Profile: | STR-PCR Data: Amelogenin: X CSF1PO: 10 D13S317: 12,13 D16S539: 11,12 D5S818: 10 D7S820: 9,10 THO1: 8,9 TPOX: 9,12 vWA: 15,18 |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 44hrs. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |