KYAE-1 cell line
SIGMA/11012002
Product Type: Chemical
| biological source | human esophagus |
| growth mode | Adherent |
| karyotype | Not specified |
| morphology | Epithelial |
| products | P53 mutation c.455C>T |
| Quality Level | 100  |
| receptors | Not specified |
| relevant disease(s) | cancer |
| shipped in | dry ice |
| storage temp. | −196°C |
| technique(s) | cell culture | mammalian: suitable |
| Cell Line Origin: | Distal oesophageal adenocarcinoma, |
| Culture Medium: | RPMI-1640:Hams F12 1:1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) |
| DNA Profile: | STR-PCR Data: Amelogenin: X CSF1PO: 11 D13S317: 11,12 D16S539: 11 D5S818: 10,13 D7S820: 11,12 THO1: 6 TPOX: 8 vWA: 14 |
| Other Notes: | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. There is a requirement to change the culture medium every 2-3 days. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −196°C |
| UNSPSC | 12352200 |