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Histopaque®-1083

SIGMA/10831 - sterile-filtered, density: 1.083

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10831-100ML 100 mL
$82.90
1/EA
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45-10831-6X100ML 100 mL
$322.00
6/EA
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application(s) hematology
histology
density 1.083
form liquid
impurities endotoxin, tested
Quality Level 400 
shelf life Expiry date on the label.
sterility sterile-filtered
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
Application: Histopaque®-1083 has been used for the isolation of mononuclear cells and leukocytes from rat peripheral blood.
Biochem/physiol Actions: Anticoagulated blood is layered onto HISTOPAQUE1083. During centrifugation, erythrocytes are aggregated by polysucrose and rapidly sediment; whereas, lymphocytes and other mononuclear cells remain at the plasma-HISTOPAQUE 1083 interface. Erythrocytes pellet to the bottom of the centrifuge tube.
Features and Benefits: Histopaque®-1083 offers numerous advantages, such as:

•  Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
•  Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light
General description: Histopaque®-1083 is a solution containing polysucrose and sodium diatrizoate, adjusted to a relative density of 1.083 g/mL. It facilitates the recovery of viable mononuclear cells from small volumes of blood of rats, mice, and other small mammals.
Legal Information: Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Density 1.083
Storage Temp. 2-8°C
UNSPSC 12352207

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