Aprotinin from bovine lung
SIGMA/10820 - lyophilized, ~80% (HPCE), crystalline (fine), white, ≥3500 U/mg
Synonym: BPTI; Bovine pancreatic trypsin inhibitor; Trasylol; Trypsin inhibitor (basic)
CAS Number: 9087-70-1
Empirical Formula (Hill Notation): C284H432N84O79S7
Molecular Weight: 6511.44
EC Number: 232-994-9
MDL Number: MFCD00130541
Linear Formula: C284H432N84O79S7
Product Type: Chemical
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung. Aprotinin is a single peptide chain with three disulfide bonds. Molecular Weight: ~6511
| assay | ~80% (HPCE) |
| biological source | bovine lung |
| color | white |
| form | crystalline (fine) |
| InChI key | ZPNFWUPYTFPOJU-UHFFFAOYSA |
| mol wt | ~6,500 |
| quality | lyophilized |
| Quality Level | 200  |
| solubility | phosphate buffer: 5 mg/mL, clear, colorless (PH 7.6 (70 mm phosphate)) |
| specific activity | ≥3500 U/mg |
| storage temp. | 2-8°C |
| technique(s) | electrophoresis: suitable |
| UniProt accession no. | P00974  |
| Application: | Aprotinin is largely used as an inhibitor of trypsin. |
| Biochem/physiol Actions: | Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges. |
| Unit Definition: | 1 U corresponds to 1 biological kallikrein inhibitor unit (KIU) |
| Unit Definition: | One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-argini |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ~80% (HPCE) |
| activity | specific activity: ≥3500 U/mg |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |